Cortisol is the major adrenal steroid hormone and is controlled
by the pituitary gland and the hypothalamus. The body’s stress response
increases cortisol in order to mobilize energy to manage and resolve
Chronic stress can fatigue the adrenal gland which can disrupt
its normal diurnal control over cortisol. This disruption can lead to
symptoms like fatigue, weight gain, insomnia, depression, and anxiety.
So if you’re feeling tired and stressed out, check your cortisol level.
Cortisol levels rise and fall during the day. The highest levels
typically occur at about 6 a.m. to 8 a.m. and the lowest levels at about
How to collect a 24-hour urine specimen:
- Do not urinate directly into the container. Urine should be
collected in another clean container and then carefully poured into the
24 hour collection container.
- Empty bladder completely on awakening in the morning and discard
this urine specimen. Record date and time under "start" and begin the
test. (i.e. 6/22/14 @ 7:00 a.m.)
- All urine passed during the rest of the day and night for the next 24 hours must be poured into the container.
- Make final collection the next morning at the exact time recorded
under start below. Record the date and time under "finish". (i.e.
6/23/14 @ 7:00 a.m.)
- Take the 24 hour specimen to the physician's office or laboratory as soon as possible.
Fasting is not required for this test. Take all medications as prescribed.